- “hydrophilic” C18
- for 100% aqueous applications
- for the separation of highly polar compounds, including metabolites and nucleotides
|
|
|
|
| YMC*Gel ODS-AQ |
Specification |
| Pore size / nm |
12 |
20 |
| Particle size / µm |
3; 5; 10; 15; 20; 50 |
3; 5; 15 |
| Surface area / m2g-1 |
330 |
175 |
| Carbon content / % |
14 |
10 |
| Recommended pH range |
2.0 - 7.5 |
2.0 - 7.5 |
Note: Analytical grades (3 µm and 5 µm) are not available as bulk materials; they are only available in prepacked columns. |
General
YMC*Gel ODS-AQ is a C18 reversed phase silica based HPLC packing material specifically designed for use in 100% aqueous eluents. As a result of the proprietary derivatisation process, YMC*Gel ODS-AQ exhibits a different selectivity to that of traditional C18 stationary phases. This difference in selectivity of YMC*Gel
ODS-AQ can be used to advantage for HPLC separations, which are difficult to achieve with conventional
C18 columns.
Properties
The proprietary YMC derivatisation process creates the different selectivity of YMC*Gel ODS-AQ, where:
1. The activity of acidic unreacted silanols is reduced, allowing moderately basic compounds to be eluted with little or no peak tailing.
2. The balanced hydrophilic/lipophilic nature of the YMC*Gel ODS-AQ stationary phase leads to strong retention of polar sample solutes even in aqueous eluents.
These properties of YMC*Gel ODS-AQ are beneficial for the separation of polar organic compounds, which tend not to be retained or are unresolved when conventional C18 columns are used.
Many conventional ODS packings lose their ability to retain polar compounds in these high aqueous content mobile phases as shown below. They appear less lipophilic with densely folded C18 chains.
However, in similar mobile phases, YMC*Gel ODS-AQ maintains its brush-like C18 chain structure and its lipophilic properties and provides excellent retention of polar compounds.
Applications
YMC*Gel ODS-AQ is able to resolve compounds with minor differences in polarity from closely related chemical structures. As a result, YMC*Gel ODS-AQ is an excellent tool for the separation of drugs and
corresponding metabolites, pesticides and degradation products, or peptides and protein digests etc.
This capability of “polar recognition” opens up a broad application range for YMC*Gel ODS-AQ in life sciences and pharmacology.
Genuine linear scale-up from analytical to large scale separations is easily achievable with YMC products such as YMC*Gel ODS-AQ, where particle sizes from 3 to 150 μm are available in large lot sizes up to several hundred kilograms, if needed. This, together with the outstanding selectivity of YMC*Gel ODS-AQ, make it an essential tool to enhance the productivity of large scale chromatographic processes.
Comparison of ODS-AQ vs. conventional ODS
Conventional ODS in
standard organic mobile phases
|
Conventional ODS in
100% aqueous mobile phases |
 |
 |
|
Column: YMC*Gel ODS-A
Dimension: 250 x 10 mm
Particle Size: 20 μm
Mobile Phase: 100% water
Compounds: Highly polar and very water soluble |
YMC*Gel ODS-AQ in
standard organic mobile phases
|
YMC*Gel ODS-AQ in
100% aqueous mobile phases |
 |
 |
|
Column: YMC*Gel ODS-AQ
Dimension: 250 x 10 mm
Particle Size: 20 μm
Mobile Phase: 100% water
Compounds: Highly polar and very water soluble |
|
Start
